KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-LGG-1 | Ke et al, 202071 | N/A |
| Rabbit anti-Adh1 (for yeast ADH1 western blotting) | Rockland Immunochemicals | Cat #: 200–4143-0100 |
| Mouse anti-tubulin | DSHB | Cat #: 4A1-s |
| Rat anti-alpha-tubulin | Invitrogen | Cat #: MA1–80017 |
| Alexa Fluor 800cw goat anti-rabbit | Li-cor | Cat #: 926–32211 |
| IRDye 800cw goat anti-mouse | Li-cor | Cat #: 92632210 |
| HRP-conjugated anti-rabbit IgG | Promega Corporation | Cat #: W4018 |
| HRP-conjugated anti-rat IgG | Abcam | Cat #: ab6734 |
| Alexa Fluor 594 goat anti-rabbit | Invitrogen | Cat #: A11012 |
| Bacterial and Virus Strains | ||
| E. coli strain OP50 | CGC | N/A |
| E. coli strain XU363 | CGC | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 5-fluoro-2-deoxyuridine (FUDR) | Research Products International | Cat #: F10705–1.0 |
| Chloroquine Diphosphate Salt | Sigma-Aldrich | Cat #: C6628 |
| Cyanamide | Sigma-Aldrich | Cat #: 187364–5G |
| Glycerol | Fisher BioReagents | Cat #: BP229–1 |
| Allyl alcohol | Thomas Scientific | Cat #: C984Z15/240532–100ML |
| Carbenicillin, Disodium salt | Genesee Scientific | Cat #: 25–532 |
| TRI Reagent | Molecular Research Center | Cat #: TR 118 |
| FastSYBR Mixture | CoWin Biosciences, Inc. | Cat #: CW0955H |
| Intercept (PBS) Blocking Buffer | Li-cor | Cat #: 927–70001 |
| Critical Commercial Assays | ||
| ReadiUse TCA Deproteinization Sample Preparation Kit | AAT Bioquest | Cat #: 19501 |
| Glycerol assay kit | R-Biopharm | Cat #: NC9662370 |
| BCA protein assay kit | Fisher Scientific | Cat #: 23227 |
| Deposited Data | ||
| RNA-Seq of human adipose tissue after long term caloric restriction | Armenise et al, 201776 | Accession number: GSE95640 |
| DNA microarray of human adipose tissue after long term caloric restriction | Capel et al, 200877 | PMID: 18782868 |
| DNA microarray of human adipose tissue after long term caloric restriction | Tareen et al, 202078 | Accession number: GSE77962 |
| DNA microarray of human adipose tissue after long term caloric restriction | Capel et al, 200979 | Accession number: GSE11975 |
| DNA microarray of human adipose tissue after long term caloric restriction | Mutch et al, 201180 | Accession number: GSE24432 |
| DNA microarray of human adipose tissue after long term caloric restriction | Nookaew et al, 201381 | Accession number: GSE35710 |
| DNA microarray of mouse heart tissue after short term caloric restriction | Noyan et al, 201582 | Accession number: GSE68646 |
| DNA microarray of mouse adipose tissue after short term caloric restriction | Kim et al, 201683 | Accession number: GSE60596 |
| DNA microarray of mouse adipose tissue after long term caloric restriction | Higami et al, 200484 | PMID: 14688200 |
| DNA microarray of mouse liver tissue after long term caloric restriction | Mitchell et al, 201685 | Accession number: GSE81959 |
| DNA microarray of mouse liver tissue after short term caloric restriction | Tsuchiya et al, 200486 | PMID: 15039484 |
| DNA microarray of rat liver tissue after long term progressive caloric restriction | Pohjanvirta et al, 200887 | Accession number: GSE9121 |
| DNA microarray of mouse intestinal tissue after long term fasting | Hakvoort et al, 201188 | Accession number: GSE24504 |
| DNA microarray of mouse gastrocnemius muscle after long term caloric restriction | Lanza et al, 201289 | Accession number: GSE36285 |
| DNA microarray of mouse kidney tissue after long term fasting | Jongbloed et al, 201490 | Accession number: GSE52982 |
| DNA microarray of mouse cochlea after long term caloric restriction | Someya et al, 200691 | Accession number: GSE4786 |
| DNA microarray of mouse liver tissue after long term caloric restriction | Aon et al, 202092 | Accession number: GSE124294 |
| Experimental Models: Organisms/Strains | ||
| C. elegans strain: N2 Bristol | CGC | N/A |
| C. elegans strain: adh-1 (ok2799) | CGC | N/A |
| C. elegans strain: mxl-3 (ok1947) | CGC | N/A |
| C. elegans strain: atg-18 (gk378) | CGC | N/A |
| C. elegans strain: eat-2 (ad456) | CGC | N/A |
| C. elegans strain: OP433 [hlh-30::TY1::EGFP::3xFLAG + unc-119(+)] | CGC | N/A |
| C. elegans strain: MAH235 (sqIs19 [hlh-30p::hlh-30::GFP + rol-6(su1006)]) | CGC | N/A |
| C. elegans strain: MAH240 (sqIs17 [hlh-30p::hlh-30::GFP + rol-6(su1006)]) | CGC | N/A |
| C. elegans strain: PHX2365 gene knock-in (adh-1P::ADH-1::wrmScarlet::adh-1 3’UTR) | This study | N/A |
| C. elegans strain: GMW20 integrated arrays backcrossed 3x (adh-1P::ADH-1::wrmScarlet::adh-1 3’UTR) | This study | N/A |
| C. elegans strain: GMW21 integrated arrays backcrossed 3x (adh-1P::ADH-1::wrmScarlet::adh-1 3’UTR) | This study | N/A |
| C. elegans strain: GMW22 integrated arrays backcrossed 3x (adh-1P::ADH-1::wrmScarlet::adh-1 3’UTR) | This study | N/A |
| C. elegans strain: XD3971 (xdIs143[Pdaf-22::PLIN1::GFP; rol-6(su1006)]) | Liu et al, 201431 | N/A |
| Yeast strain: Y15090 (MATa/α [HAP1-NatMX-ACT1pr-Z3EV-ENO2term]/HAP1 ura3∆0/URA3 [can1∆::STE2pr-SpHIS5]/CAN1 his3∆1/his3∆1 lyp1∆/LYP1) | Calico labs | N/A |
| Oligonucleotides | ||
| Primers for qRT-PCR, see Table S1 | This study | N/A |
| Software and algorithms | ||
| SPSS | IBM | N/A |
| GraphPad Prism | GraphPad | N/A |
| OASIS 2 | Han et al, 201670 | N/A |