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. 2023 May 2;299(6):104774. doi: 10.1016/j.jbc.2023.104774

Figure 4.

Figure 4

Oncogenic properties of deregulated K2.A, normal breast epithelial MCF10A cells were transfected with K2-WT or K2-DM, embedded in 2% Matrigel, and 3D acini were imaged by bright-field microscopy after 4 and 7 days. Representative images. The scale bar represents 100 μm. Insets, magnification of indicated areas. The scale bar represents 50 μm. B and C, the conditions are as in (A), and 3D acini surface area (B) and circularity (C) were quantified (mean to max, n = 33–60). D and E, Three-dimensional acini as in (A) were harvested after 8 days or 14 days (vimentin) and analyzed by immunofluorescence microscopy (D, representative images) with quantification of the percentage of Ki-67 (E, top)-, cleaved (Cl) caspase-3 (E, middle)-, and vimentin (E, bottom)-positive cells. MFI, mean fluorescence intensity (min to Max, n = 6–7). The scale bar represents 10 μm. For all panels, numbers correspond to p values by one-way ANOVA with Tukey multiple comparisons test.