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. 2023 May 2;299(6):104767. doi: 10.1016/j.jbc.2023.104767

Supporting Figure S2.

Supporting Figure S2

A, effect of ASFV-ΔMGF360-9L or ASFV-ΔMGF110-9L on Pam3CSK4-triggered phosphorylation of IκBα or p65 in primary porcine alveolar macrophages (PAMs). PAMs were infected with ASFV-ΔMGF360-9L or on Pam3CSK4-triggered phosphorylation of (MOI = 0.1) for 6, 12, and 18 h or uninfected (mock), and then the cells were treated or not with Pam3CSK4 (100 ng/ml) for another 4 h. The cell lysates were analyzed by immunoblotting utilizing the indicated antibodies. B, effect of TLR2 on TLR2 signaling in vitro. The iPAM cells were transfected with HA-TLR2 plasmid (0, 1, 2 and 4 μg) for 20 h, and then treated with Pam3CSK4 (100 ng/ml) for another 4 h. The cell lysates were analyzed by immunoblotting utilizing the indicated antibodies.