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. 2023 May 2;299(6):104767. doi: 10.1016/j.jbc.2023.104767

Figure 1.

Figure 1

Construction of ASFV-ΔMGF110/360-9L.A, schematic representation of the gene(s) and region(s) deleted in each gene-deleted ASFV. The deleted gene segments were replaced with the p72 eGFP or p72 mCherry reporter gene cassette as indicated. Nucleotide positions indicating the boundaries of the deletion relative to the ASFV CN/GS/2018 genome are indicated. B, fluorescence of virus-infected primary porcine alveolar macrophages. Scale bar represents 300 μm. C, PCR analysis of ASFV-ΔMGF110/360-9L using specific primers targeting MGF110-9L, MGF360-9L, or p72 (B646L) genes. ∗, nonspecific band. D, multistep virus growth curves of ASFV-ΔMGF110-9L, ASFV-ΔMGF360-9L, ASFV-ΔMGF110/360-9L, and parental ASFV CN/GS/2018. Primary swine macrophage cell cultures were infected (MOI = 0.1) with each of the viruses and virus titers at the indicated times postinfection. The graphs show the mean ± SD. In the displayed experiment, three replicates were included per experimental condition. MOI, multiplicity of infection; ASFV, African swine fever virus.