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. 2023 May 2;299(6):104767. doi: 10.1016/j.jbc.2023.104767

Figure 7.

Figure 7

Effect of Toll-like receptor 2 on ASFV titer and ASFV p72 protein.A and B, MA-104 cells were transfected with TLR2 plasmid for 24 h, followed by infection with ASFV for the indicated time. A, ASFV titer and (B) ASFV p72 expression level in TLR2-overexpressed MA-104 cells. The graphs show the mean ± SD. In the displayed experiment, three replicates were included per experimental condition. ∗p < 0.05, represent the EV- and TLR2-overexpressed groups. C–E, primary porcine alveolar macrophages (PAMs) were transfected with control-RNAi or TLR2-RNAi by Lipofectamine 2000 for 48 h. PAMs were infected with ASFV (MOI = 0.1) for the indicated times followed by RT-PCR analysis. C, TLR2 levels in TLR2-knockdown PAMs infected with ASFV. The graphs show the mean ± SD. In the displayed experiment, three replicates were included per experimental condition. ∗p < 0.05, represent the control-RNAi– and TLR2-RNAi–silenced groups. D, ASFV titer of TLR2-knockdown PAM cells. The graphs show the mean ± SD. In the displayed experiment, three replicates were included per experimental condition. ∗p < 0.05, represent the control-RNAi– and TLR2-RNAi–silenced groups. E, ASFV p72 protein in TLR2-knockdown PAMs. ASFV, African swine fever virus; RT-PCR, real time PCR; RNAi, interfering RNA; MOI, multiplicity of infection; TLR2, Toll-like receptor 2.