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. 2023 May 4;299(6):104784. doi: 10.1016/j.jbc.2023.104784

Figure 4.

Figure 4

Leptin-deficient black ob/ob mice and WT black C57BL/6J mice. Mouse cohorts were gender matched (female/male). A, retinol binding protein 4 (Rbp4) in the plasma of ob/ob mice aged 5 months and C57BL/6J mice aged 4 months. Measurements were carried out by ELISA. Data are normalized as fold change in Rbp4 in ob/ob mice relative to C57BL/6J, n = 6 to 10. p value was determined by unpaired two-tailed t test. B, retinoid levels in the eyes of light adapted 2 months-old ob/ob and C57BL/6J mice. atROL, all-trans-retinol; atRE, all-trans-retinyl ester; all-trans-retinal (atRAL), 11-cis-retinal (11cisRAL). Retinoid extraction was performed using method 2. Values were obtained from single eyes and are plotted together with mean ± SD, n = 8 to 10. p values were determined by two-way ANOVA and Sidak’s multiple comparison test. C, quantitation of bisretinoids in ob/ob and C57BL/6J mice at ages 2, 4, 6, and 8 months. Individual values and mean ± SD are plotted, n = 2 to 5 replicates. Extraction method B was used. P values determined by two-way ANOVA and Sidak’s multiple comparison test. D and E, quantitation of SW-AF (short-wavelength fundus autofluorescence; 488 nm excitation) and NIR-AF (near-infrared fundus AF; 790 nm excitation) at age 2, 4, 6, and 8 months. Individual values and mean ± SD are plotted, n = 3 mice. Means were not significantly different (one-way ANOVA and Tukey’s multiple comparison test). F, outer nuclear layer (ONL) thickness in ob/ob mice and C57BL/6J mice at ages 2, 4, 6, 8, and 12 months. ONL thickness is plotted as distance from optic nerve head (ONH), n = 6 to 12 eyes. G, ONL area calculated from ONL thickness measurements as described in Experimental procedures. Values are mean ± SD. p values were determined by one-way ANOVA and Tukey’s multiple comparison test. n = 6 to 12 eyes.