FIGURE 5.

Transcriptional control of fasting and weight loss and its regulation by Crtc2. (A) Mice tibialis anterior (TA) muscle were transduced with GFP control or Crtc2 expression vector. Crtc2 mRNA levels in the TA of mice fed ad libitum (Fed), or subject to three cycles of ADF (Fast) were compared by qPCR. N = 3 mice per group. (B) Venn diagram showing the numbers of DEGs identified by mRNA-seq comparing the effects of ADF in control versus Crtc2-transduced TA muscles. (C) Biological processes and functions regulated by ADF and Crtc2. Gene ontology (GO) analysis suggests that ADF regulates several processes/functions in a Crtc2-sensitive manner. The numbers of DEGs involved in each process are shown. See Supporting Information Dataset S1 for a complete list of represented GO annotations. (D) Examples of ADF-regulated transcriptional programs and mRNAs in control TA muscles. (E) Gene expression profiles in control and Crtc2-transduced TA muscles of mice subjected to ADF relative to the ad libitum fed mice (columns 1–2) , and effect of Crtc2 transduction relative to control during ADF (column 3). ADF-regulated genes in control muscle appear in bold. *Crtc2-regulated genes in mice subjected to ADF. *ADF-regulated genes in Crtc2-transduced muscle but not control. FC, fold change