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. Author manuscript; available in PMC: 2023 Jun 2.
Published in final edited form as: FASEB J. 2021 Dec;35(12):e21999. doi: 10.1096/fj.202100171R

FIGURE 6.

FIGURE 6

Protein synthesis and mTOR signaling. (A) Mice tibialis anterior (TA) muscle were transduced with GFP control or Crtc2 expression vector. Crtc2 mRNA levels in the TA of mice fed ad libitum (Fed), or subject to three cycles of ADF (Fast) were isolated and weighted. N = 8 mice per group. (B) Quantitation of western blot (Supporting Information Figure S6B) for SUnSET protein synthesis assay. Mice electroporated and fed as in (A) were fasted overnight and treated with puromycin for 30 min and then the TA muscles collected for western blot. N = 4 mice per group. (C) TA muscle were isolated from mice electroporated and fed as in (A) for western blot analyses. N = 5 per group. (D–F) Quantitation of (C)