FIGURE 5.

Circular RNA nuclear receptor‐interacting protein 1 (circNRIP1) promotes tumor growth in vivo. (A) Subcutaneous implant mouse model established after inoculation with pcDNA3.1, overexpression‐circNRIP1 (ov‐circNRIP1), ov‐circNRIP1 + mir‐541‐5p mimic, and ov‐circNRIP1 + mir‐3064‐5p mimic TPC1 cells (n = 5). (B) Tumor volumes and (C) tumor weights. (D) Quantitative real‐time PCR showing relative expressions of circNRIP1, miR‐541‐5p, miR‐3064‐5p, and pyruvate kinase M2 (PKM2) in pcDNA3.1 and ov‐circNRIP1 groups. (E) Tumor sections stained with H&E. PKM2 levels were evaluated by immunohistochemistry. (F) Representative images of¹⁸F‐fluoro‐2‐deoxyglucose (¹⁸F‐FDG) uptake in pcDNA3.1, ov‐circNRIP1, ov‐circNRIP1 + mir‐541‐5p mimic, and ov‐circNRIP1 + mir‐3064‐5p mimic xenograft mouse models. Red circles indicate tumor glucose uptake. Maximum uptake values (SUVmax) for xenografts were determined by ¹⁸F‐FDG PET. *p < 0.05, **p < 0.01