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. 2023 Jun 1;220(9):e20221751. doi: 10.1084/jem.20221751

Figure 2.

Figure 2.

Inhibition of citrullination protects from periodontal bone loss. (A and B) Bone loss measurements with or without LIP (6 d) in (A) WT and Padi4 KO (n = 5–6) mice or (B) mice treated with CI-amidine or vehicle (n = 10). (C) Flow cytometry analysis of mouse oral gingival tissues after LIP (18 h) in WT and Padi4 KO mice (n = 4). Graph indicating absolute number of CD45+ or neutrophils (CD45+CD11b+CD11clow/medLy6G+) per standardized tissue block. (D–H) Immunofluorescence for citH3, Ly6G, MPO in gingival tissues from Padi4 KO and WT mice, with or without LIP (n = 5, 18 h). (D and G) Representative images and conditions are indicated. Scale bars are 50 µm. (E, F, and H) Quantification, mean fluorescence intensity for citH3, Ly6G, and MPO staining (n = 5). Data are representative of three (A, C, E, F, and H) or four (B) independent experiments. Graphs show the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. One-way ANOVA with Tukey’s multiple comparison test (A, B, E, F, and H), unpaired t test (C).