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. 2023 Jun 1;220(9):e20221751. doi: 10.1084/jem.20221751

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© 2023 Moutsopoulos et al.

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Figure S2. — Citrullination of NETs potentiates inflammatory bone destruction. (A and B) Representative TRAP-stained sections of gingival tissues with or without LIP (6 d) in WT (n = 5) and Padi4 KO (n = 5) mice. (A) TRAP-positive cells appear as purplish to dark red in the sections. Scale bars are 50 µm; representative image. (B) Quantification of osteoclasts per condition, number of osteoclast/bone surface (N/mm). (C–H) Flow cytometry analysis of mouse oral gingival tissues with or without LIP (18 h) in WT (n = 4) and Padi4 KO (n = 4) mice. Graph indicating absolute number of CD45⁺ (C), neutrophils (CD45⁺CD11b⁺CD11c^low/medLy6G⁺; D), lymphoid DCs (CD11b⁻CD11c⁺; E), myeloid DCs (CD11b⁺ CD11c^low-med; F), MNPs (CD11b⁺CD11c^low-medLy6G⁻Ly6C^low-highSSC^low; G), and eosinophils (CD11b⁺CD11c^low-medLy6G⁻Ly6C^low-neg SSC^high; H) per standardized tissue block. Data are representative of three (B–H) independent experiments. Graphs show the mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001. One-way ANOVA with Tukey’s multiple comparison test (B), unpaired t test (C–H).