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. 2023 Jun 1;220(9):e20221751. doi: 10.1084/jem.20221751

Figure S3.

Figure S3.

Histones meditate IL-17 cell accumulation in periodontitis. (A–C) Western blot analysis for H3, H4, citH3, and β-actin in mouse oral gingival tissues with or without LIP (6 d) in isotype IgG H3 (n = 5), (A) anti-H3 (n = 5), (B) anti-H4 (n = 5), or (C) anti-citH3 treated mice (n = 5). (D–F) Flow cytometry analysis of mouse oral gingival tissues with or without LIP (4 d) in isotype IgG (n = 5) and anti-citH3 (n = 5) treated IL-17acreR26ReYFP mice. (D) FACS plot and graphs indicating (E) numbers and (F) percentage of γδT+eYFP+, CD8eYFP+, as well as CD3−eYFP+ cells. Data are representative of three (A–C, E, and F) independent experiments. Graphs show the mean ± SEM. ***P < 0.001, ****P < 0.0001. One-way ANOVA with Tukey’s multiple comparison test (E and F). Source data are available for this figure: SourceData FS3.