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. 2023 Jun 1;220(9):e20221751. doi: 10.1084/jem.20221751

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© 2023 Moutsopoulos et al.

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Figure S4. — NETs support IL-17 cell accumulation in periodontitis. (A and B) Flow cytometry analysis of IL-17a^creR26R^eYFP mice oral gingival tissues after LIP (4 d) treated with DNase-I (n = 4) or vehicle (n = 4). Graphs indicating the percentage of (A) CD45^+eYFP+ or (B) CD4^+eYFP+ cells. (C and D) Flow cytometry analysis of mouse oral gingival tissues in CTL/LIP mice at the indicated times (n = 3, 0–120 h). Graphs indicating numbers of (C) CD45⁺ or (D) CD4⁺IL-17⁺ cells. (E and F) Flow cytometry analysis in WT mice with or without LIP (n = 4, 4 d). Graph indicating percentage of (E) CD45⁺ or (F) CD4⁺TLR2⁺ cells. (G) 16S rRNA sequencing of LIP microbial communities from DNase-I and vehicle-treated mice at 18 h and 6 d (n = 7–8). Relative abundance of most abundant bacteria, OTU level. (H and I) Total oral microbial biomass in WT (n = 4) and Padi4 KO (n = 4) mice at (H) 18 h and (I) 6 d after LIP. Data are representative of three (A–F, H, and I) independent experiments. Graphs show the mean ± SEM. *P < 0.05. ns, not significant. One-way ANOVA with Tukey’s multiple comparison test (C and D), Unpaired t test (A, B, E, F, H, and I).