. 2023 May 29;16:3305–3313. doi: 10.2147/IDR.S410914

Table 3.

Accuracy of Metagenomic Next Generation Sequencing and Culture for Spinal Tuberculosis Compared with Composite Reference Standard

Test		CRS		Sensitivity (%)	Specificity (%)	PPV (%)	NPV (%)	AUC
Test		Positive	Negative	Sensitivity (%)	Specificity (%)	PPV (%)	NPV (%)	AUC
mNGS	Positive	79	0	71.2 (61.8–79.4)	100.0 (96.1–100.0)	100.0 (95.4–100.0)	74.2 (65.5–81.6)	0.86 (0.80–0.90)
mNGS	Negative	32	92	71.2 (61.8–79.4)	100.0 (96.1–100.0)	100.0 (95.4–100.0)	74.2 (65.5–81.6)	0.86 (0.80–0.90)
Culture	Positive	81	0	73.0 (63.7–81.0)^a	100.0 (96.1–100.0)	100.0 (95.6–100.0)	75.4 (66.8–82.8)^a	0.86 (0.81–0.91)^a
Culture	Negative	30	92	73.0 (63.7–81.0)^a	100.0 (96.1–100.0)	100.0 (95.6–100.0)	75.4 (66.8–82.8)^a	0.86 (0.81–0.91)^a

Notes: Compared mNGS and culture, ^aP>0.05.

Abbreviations: CRS, composite reference standard; PPV, positive predictive value; NPV, negative predictive value; AUC, area under the curve; mNGS, metagenomic next generation sequencing.