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. 2023 Jun 2;18(6):e0285971. doi: 10.1371/journal.pone.0285971

Fig 1. HEK293-derived transient and stable pool expression results in much less clipping than observed in CHO-K1 cells.

Fig 1

(A) Protein 1 with a C-terminal Fc tag expressed from CHO-K1 stable pools (left panel) and Expi293F transients (middle panel) were separated by SDS-PAGE under non-reducing (NR) and reducing conditions (R) and stained by Coomassie blue. Analytical SEC analysis of CHO-K1 stably and Expi293F transiently-expressed protein 1 is shown in the right panel. (B) As in (A), but with protein 2 with a C-terminal Fc tag. (C) As in (A), but with protein 3 with a C-terminal His6 tag expressed from CHO-K1 stable pools, Expi293F transient expression and Expi293F stable pools (left panels). Analytical SEC of CHO-K1 stably, Expi293F transiently and stably-expressed protein 3 (middle panel) and their zoom-in traces (right panel) are also shown. (D) As in (A), but with protein 4 with a C-terminal His6 tag expressed from CHO-K1 stable pools (left panel) and Expi293F stable pools (middle panel). Protein 4 expressed from Expi293F stable pools were subject to PNGase F digestion and analyzed by SDS-PAGE. Analytical SEC showing the migration profile of CHO-K1 and Expi293F stably-expressed protein 4 (right panel).