Table 1.
Cryo-EM single particle data collection and refinement statistics.
| PfCSP in complex with L9 (Class 1) | PfCSP in complex with L9 (Class 2) | |
|---|---|---|
| EMDB ID | 28192 | 28196 |
|
| ||
| PDB ID | 8EK1 | 8EKA |
|
| ||
| Data Collection | ||
| Microscope | FEI Glacios | FEI Glacios |
| Voltage (kV) | 200 | 200 |
| Electron dose (e− /Å2) | 63.7 | 63.7 |
| Detector | Gatan K3 | Gatan K3 |
| Pixel Size (Å) | 0.46 | 0.46 |
| Defocus Range (μm) | −0.3 to −2.2 | −0.3 to −2.2 |
| Magnification | 36000 | 36000 |
| Reconstruction | ||
| Software | cryoSparcV3.3 | cryoSparcV3.3 |
| Particles | 117,479 | 62,735 |
| Symmetry | C1 | C1 |
| Box size (pix) | 320 | 320 |
| Resolution (Å) (FSC0.143) | 3.64 | 3.72 |
| Refinement | ||
| Software | Phenix 1.18 | Phenix 1.18 |
| Protein residues | 1102 | 905 |
| Chimera CC | 0.83 | 0.84 |
| EMRinger Score | 2.83 | 2.26 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.004 | 0.003 |
| Bond angles (°) | 0.735 | 0.681 |
| Validation | ||
| Molprobity score | 2.04 | 2.03 |
| Clash score | 9.39 | 7.54 |
| Favored rotamers (%) | 99.47 | 100 |
| Ramachandran | ||
| Favored regions (%) | 90.31 | 87.40 |
| Allowed regions (%) | 9.23 | 12.26 |
| Outlier regions (%) | 0.46 | 0.34 |