Fig 1. Identification of HKs impacting vpsL transcription in Vibrio cholerae.

Promoter activity of the transcriptional fusion PvpsL-lux was measured from cells grown to exponential phase in strains harboring deletions of individual HKs and WT. The orange coloring indicates the HK (VCA0257) that is the focus of this study. Individual data points of Relative Luminescent Units (RLU) are plotted with bars at the mean and error bars representing standard deviation. Statistical significance was determined using a One-Way ANOVA and post-hoc Tukey’s multiple comparisons test. Means from individual biological replicates (n ≥ 3) were compared to that of wild type, and differences with an adjusted P value of ≤ 0.01 were deemed significant. ***, P ≤ 0.0001.