Fig 3. Epistasis analysis of rvv loci contributions to biofilm formation.

Promoter activity of the transcriptional fusions PvpsR-lux (A), and PvpsT-lux (B) PvpsL-lux (C, D) were measured from cells grown to exponential phase in the indicated strains. Individual data points (circles–RvvA present; triangles–RvvA deleted) of Relative Luminescent Units (RLU) are plotted with crossbars representing mean and standard deviation. (A, B), Statistical significance was determined using a One-Way ANOVA and post-hoc Tukey’s multiple comparisons test. Means from individual biological replicates (n ≥ 3) were compared to that of wild type, and differences with an adjusted P value of ≤ 0.01 were deemed significant. **, P ≤ 0.001; ***, P ≤ 0.0001. (C, D) Means from individual biological replicates (n ≥ 3) were compared by an unpaired t-test, and mean differences with a P value of ≤ 0.01 were considered significant. **, P ≤ 0.001; ***, P ≤ 0.0001; ns, not significant.