Figure 6.

Combination of DNase I and NAC reverses inflammation and oxidative stress in AILI. A) The GO functional enrichment analysis of DEGs between DNase I + NAC and Model at 24 h following APAP (250 mg kg⁻¹). B) Heat maps of significantly DEGs involved in oxidative stress and inflammation. C,D) qRT‐PCR of oxidative stress and inflammation related genes (n = 3 for each group). E) Heat maps of IHC H‐scores of TNF‐α, TGF‐β, 3‐NT, and TLR9. F) IHC staining of TLR9 at 24 h following APAP. Original magnification, ×400; Scale bar: 25 µm. G) The expression of TLR9 in neutrophils was determined by flow cytometry at 24 h following APAP. H) The activation of HEK‐Blue TLR9 reporter cells by CpG ODN 2006 was inhibited by DNase I and combination therapy. n = 3–4 for each group. Data are expressed as means ± SD, ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001.