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. 2023 Apr 17;10(16):2205993. doi: 10.1002/advs.202205993

Figure 2.

Figure 2

Adgrv1 Y6236fsX1 mutation leads to impairment of MET functions. a) FM1‐43FX dye uptake by auditory hair cells from P5 mice of different genotypes as indicated. Images were taken from the middle turn of the cochlea using a confocal microscope. Scale bar: 20 µm. Representative images from three independent experiments were shown. b,c) Relative fluorescence intensity in b) OHCs and c) IHCs from mice of different genotypes were measured and analyzed using ImageJ software. Data are correlated with Figure 2a. Data were obtained from 15–23 OHCs or 32–38 IHCs from three mice in each group. d) Representative saturating MET currents in OHC from P6 Y6236fsX1 heterozygous or homozygous mice triggered by a fluid jet system. e) Statistics of saturating MET currents in OHCs from P6 Y6236fsX1 heterozygous or homozygous mice. Data are correlated to Figure 2d. Data information: b,c) ***p < 0.001; Adgrv1/del7TM or Y6236fsX1 mutant mice compared with WT mice. e) ***p < 0.001; homozygous Adgrv1 Y6236fsX1 mice compared with heterozygous Adgrv1 Y6236fsX1 mice. The bars indicate the mean ± SEM values. Data were statistically analyzed using b,c) one‐way ANOVA with Dunnett's post‐hoc test or e) Student's t‐test.