Table 2.

Main flow control steps in a protocol for a lung-on-a-chip experiment Huh et al. (2010), Benam et al. (2016), Hancock and Elabbasi (2017)

Step	Process	Type of fluid	Flow rate
1	Conditioning of the channels and cell chamber	Ethanol, DI water	50 $\mathrm{\mu }$Lmin${}^{-1}$
2	Epithelial cell seeding on one side of the membrane in the cell chamber	Culture medium with cells	Static or 1.5 $\mathrm{\mu }$Lmin${}^{-1}$
3	Endothelial cell seeding on another side of the membrane in the cell chamber	Culture medium with cells	Static or 1.5 $\mathrm{\mu }$Lmin${}^{-1}$
4	Cell culture	Culture medium	1.5-50 $\mathrm{\mu }$Lmin${}^{-1}$
5	Emulating breathing by cyclic stretching of the membrane.	Air, Vacuum	0.15-0.25 Hz, 10-15 % mechanical strain