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. 2023 Jun 2;14:3187. doi: 10.1038/s41467-023-38986-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Volcano plots of differential gene expression analysis between Mef2>WT and Mef>M1BP RNAi adult flight muscles. Analysed genes belong to the mitochondrial quality control response: UPR-related genes, UPS-related genes, and mitochondrial proteases. For statistical analysis, the Wald test with Benjamini–Hochberg correction was applied. b Confocal sections of wild-type (top) and M1BP RNAi (bottom) mitochondria in adult flight muscles labelled with mit::mKate2 and anti-Hsp70 antibody, to visualise a part of the unfolded protein response. White rectangles depict an enlarged view of a single, aggregate-containing mitochondrion (right). The scale bar corresponds to 10 µm. c Western blot analysis of Ubiquitin and Hsp70 on proteins obtained from adult purified mitochondria. The Ponceau S membrane staining is shown as a loading control. The asterisk represents an example of proteins with very low or no ubiquitination in the WT condition compared to the M1BP KD and the dagger a protein whose ubiquitination further increased upon M1BP KD. d Coomassie Blue staining of detergent-soluble and insoluble fractions obtained from adult flight muscles. e Western Blot analysis of Ubiquitin on detergent-soluble and insoluble fractions obtained from adult flight muscles. The Ponceau S membrane staining is shown as a loading control. Source data are provided as a Source data file.