Table 2.
Plasma lipids, HDL composition, and HDL-mediated cholesterol efflux in women with BC and CTR.
| CTR | BC | P | |
|---|---|---|---|
| n* | 132 | 126 | |
| Age (years) | 48 (37–58) | 55 (49–63) | < 0.001 |
| BMI (kg/m2) | 28 (25–31) | 27 (24–31) | 0.391 |
| TC (mg/dL) | 172 (151–199) | 178 (155–206) | 0.423 |
| TG (mg/dL) | 84 (59–122) | 92 (68–116) | 0.224 |
| apoB (mg/dL) | 111 (89–139) | 107 (82–141) | 0.428 |
| HDLc (mg/dL) | 41 (35–51) | 40 (32–48) | 0.109 |
| LDLc (mg/dL) | 111 (95–131) | 115 (95–142) | 0.522 |
| VLDLc (mg/dL) | 17 (12–24) | 18 (14–23) | 0.224 |
| non-HDLc (mg/dL) | 127 (109–156) | 136 (113–163) | 0.252 |
| TC/apoB (mg/dL) | 1,5 (1,3–1,9) | 1,7 (1,4–2,0) | 0.065 |
| TG/HDL (mg/dL) | 1,9 (1,3–3,1) | 2,3 (1,5–3,5) | 0.091 |
| HDL-TC (mg/dL) | 44 (34–55) | 37 (30–46) | 0.001 |
| HDL-TG (mg/dL) | 14 (10–19) | 13 (10–16) | 0.039 |
| HDL-PL (mg/dL) | 98 (80–131) | 87 (73–116) | 0.006 |
| HDL-apoA-I (mg/dL) | 104 (82–141) | 112 (82–142) | 0.700 |
| 24HC (ng/mL) | 38.9 (25.9–99.0) | 34.9 (24.1–65.4) | 0.203 |
| 25HC (ng/mL) | 8.1 (5.1–10.7) | 6.9 (5.4–9.3) | 0.111 |
| 27HC (ng/mL) | 12.6 (7.4–19.4) | 7.7 (4.6–13.1) | 0.002 |
| 14C-cholesterol efflux (%) | 12 (9–18) | 13 (9–19) | 0.501 |
Plasma lipids were determined by enzymatic colorimetric methods and apoB by immunoturbidimetry. HDLc was determined after precipitation of lipoproteins containing apoB; non-HDLc was calculated as TC-HDLc. Oxysterols were quantified in isolated HDL by gas chromatography-mass spectrometry (GC–MS). The cholesterol efflux was determined in BMDM overloaded with 14C-cholesterol and acetylated LDL, utilizing 50 µg/mL of HDL as a cholesterol acceptor.
BMI, body mass index; TC, total cholesterol; TG, triglycerides; apoB, apolipoprotein B; HDLc, high-density lipoprotein cholesterol; LDLc, low density lipoprotein cholesterol; VLDLc, very low density lipoprotein cholesterol; PL, phospholipids; apoA-I, apolipoprotein A-I; 24HC, 24-hydroxycholesterol (CTR, n = 82; BC, n = 82); 25HC, 25-hydroxycholesterol (CTR, n = 82; BC, n = 82); 27HC, 27-hydroxycholesterol (CTR, n = 81; BC, n = 80). Comparisons were made using the Mann–Whitney test; data shown in median and interquartile ranges (25–75%). P values were age-adjusted.