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. 2023 May 18;20:100667. doi: 10.1016/j.mtbio.2023.100667

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© 2023 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — The biocompatibility and osteogenic capability of BMSCs on scaffolds. (A) Cell proliferation of BMSCs cultured on Gyroid, Cylindrical, Cubic pore scaffolds and Control evaluated by CCK-8 kit on days 1, 3, and 7. (B) Representative ALP staining image of BMSCs cultured on Gyroid, Cylindrical, Cubic pore scaffolds and Control in the osteogenic medium after culturing for 7 days. Scale bar = 200 μm. (C) Western blot images of OPN, and Runx-2 protein expression in BMSCs cultured on different scaffolds. (D) qRT-PCR analysis the relative mRNA expression levels of Col-1, OCN, and Runx-2 in BMSCs cultured on Gyroid, Cylindrical, Cubic pore scaffolds and Control for 7 days. The control group received only the culture medium. Data represented as mean ± SD (n = 3, ∗p < 0.05, ∗∗p < 0.01).