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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: Nat Chem Biol. 2023 Feb 6;19(6):719–730. doi: 10.1038/s41589-022-01249-3

Figure 1. Exogenous deuterated polyunsaturated fatty acids rescue against ferroptosis and accumulate perinuclearly and in puncta.

Figure 1.

A. Structures of polyunsaturated fatty acids deuterated at their bis-allylic sites. B. Rescue of HT-1080 cells treated with each of four classes of ferroptosis inducers after a 24-hour pre-treatment with D-PUFAs. Data are represented as mean ± SEM, n=3. C. SRS images of HT-1080 cells treated for 24 hours with 20 μM DHA-d10. The protein (CH3) and lipid (CH2) cell-intrinsic vibrational frequencies are shown as well for comparison. Red arrow points to lipid droplets and white arrow points to perinuclear accumulation. D. SRS imaging of HT-1080 cells treated for 24 hours with the three different D-PUFAs used in this study, ARA-d6 (80 μM), EPA-d8 (20 μM), and DHA-d10 (20 μM). Red arrow points to lipid droplets and white arrow points to perinuclear accumulation. E. SRS imaging of Panc-1, N27, and HT-22 cells treated for 24 hours with 20 μM DHA-d10.