Extended Data Fig. 6. FINO₂-0 and FINO₂-2 accumulate in the ER, and FINO₂-1/FINO₂-3/FINO₂-4 do not accumulate in the plasma membrane.

A. Structure of FINO₂-0. B. Dose-response curve of HT-1080 cells treated with FINO₂-0 ± fer-1. Data are represented as mean ± SEM, n=3. C. Confocal fluorescence images of HT-1080 cells treated for 3 hours with 3 μM FINO₂-1 alone or with 3 μM fer-1. D. Confocal fluorescence image of HT-1080 cells treated with 3 μM FINO₂-0. F. Confocal fluorescence imaging of HT-1080 cells treated with FINO₂-1 (3 μM) and fer-1 (3 μM) for 3 hours, co-stained with BODIPY TR ceramide. G. Structure of FINO₂-2. H. Dose-response curve of HT-1080 cells treated with FINO₂-2 ± fer-1. Data are represented as mean ± SEM, n=3. I. SRS and fluorescence imaging of HT-1080 cells treated with 20 μM FINO₂-2 and 2 μM fer-1, and stained with Nile Red and Lysotracker green. J. Confocal fluorescence image of HT-1080 cells treated with 3 μM FINO₂-1 and 3 μM fer-1, and costained with CellMask Deep Red. K. Confocal fluorescence image of HT-1080 cells treated with 3 μM FINO₂-3 or 3 μM FINO₂-4, and 3 μM fer-1, and co-stained with CellMask Deep Red.