Skip to main content
. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: Nat Chem Biol. 2023 Feb 6;19(6):719–730. doi: 10.1038/s41589-022-01249-3

Figure 3. Anti-ferroptotic D-PUFAs incorporate into ER phosphatidylethanolamine phospholipids and ether phospholipids.

Figure 3.

A. SRS and fluorescence imaging of HT-1080 cells treated with DHA-d10 (20 μM) ± co-treatment with DGAT inhibitors PF-06424439 (1 μM) and A922500 (1 μM), and then stained with ERTracker Green. B. SRS and fluorescence imaging of HT-1080 cells treated with DHA-d10 (20 μM) and then stained with ERTracker Green and BODIPY TR ceramide (a Golgi stain). White arrows indicate the Golgi region. C. Heatmap showing incorporation of deuterated DHA into HT-1080 phospholipids as measured by LC-MS. Vehicle shows no deuterated incorporation, whereas DHA-d10 has varying incorporation depending on the lipid species and fatty acid compositions. PE: phosphatidylethanolamine, PI:phosphatidylinositol, PS:phospatidyserine, PC: phosphatidylcholine. Data shown are an average of absolute signal intensity of three biological replicates.