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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: Cancer Prev Res (Phila). 2023 Jun 1;16(6):321–332. doi: 10.1158/1940-6207.CAPR-22-0384

Figure 1. The treatment regimen of suberoylanilide hydroxamic acid (SAHA) and lipopolysaccharide (LPS) for metabolomics and next-generation sequencing (NGS) analysis.

Figure 1.

(A) The treatment regimen for metabolomic analysis by LC-MS; the red lines indicated the group of 3 h treatment of SAHA followed by 24 h treatment of LPS. The blue line means the group of 24 h treatment of SAHA followed by 24 h treatment of LPS. After the treatment of LPS, all two groups underwent the harvesting of cells and LC-MS analysis. (B) Treatment regimen for NGS analysis; the blue line means the group of 5-day treatment of SAHA followed by 1-day treatment of LPS. After LPS treatment, all two groups underwent the harvest of cells and the analysis of NGS.