Figure 2. Metabolic analysis of suberoylanilide hydroxamic acid (SAHA) treated on lipopolysaccharide (LPS)-challenged BEAS-2B cells.
(A) Cell viability of BEAS-2B cells treated with SAHA and challenged with 1 μg/mL LPS; (B) Principal component analysis (PCA) plot of metabolites after SAHA treatment for 3 and 24 h; (C) Significant enrich pathway analysis; (D) Heatmap of metabolites associated with nicotinate and nicotinamide pathway, methionine metabolism, and glutathione pathway after 3 h treatment; (E) Heatmap of metabolites associated with nicotinate and nicotinamide pathway, methionine metabolism, and glutathione pathway after 24 h treatment. All experiments were conducted in triplicate (n = 3).