FIGURE 1.

16HBE14o⁻ cell line contains two subpopulations expressing differing amounts of CFTR transcript and protein. (a) Schema illustrates the generation of 16HBE14o⁻ clones from single cells following manual dilution and subsequent analyses. (b) CFTR expression normalized to β2M, shown relative to 16HBE14o⁻ WT cells (n = 2). Cells at two non‐sequential passages were evaluated for 12 clones, with the four identified for subsequent analyses shown in brown (CFTR^high) or blue (CFTR^low). ** denotes p < 0.01 and * denotes p < 0.05, compared to 16HBE14o⁻ using unpaired t‐tests with Welch's correction. (c) Western blot showing CFTR protein levels with an antibody specific for CFTR in 16HBE14o⁻ cells and two clones each of CFTR^high and CFTR^low cells. β‐tubulin provides the loading control. The data shown are representative of the two separate passages surveyed.