FIGURE 4.

Transcriptomic differences of CFTR^high and CFTR^low cells. (a) Principal component analysis (PCA) plot comparing RNA‐seq data from individual replicates of CFTR^high and CFTR^low clones (n = 3 of each). (b) Volcano plot of RNA‐seq data comparing CFTR^high and CFTR^low cells (−log₁₀ adjusted p‐value vs. log₂ fold change) with genes upregulated in CFTR^low and in CFTR^high cells shown to the left and right, respectively. Shown in red are genes with an absolute fold change of ≥1.5 and an adjusted p‐value of ≤0.01. (c,d) Gene ontology process enrichment analysis of 311 genes upregulated in CFTR^high cells (c) and 169 genes upregulated in CFTR^low cells (d), when comparing CFTR^high and CFTR^low. Terms with a p‐value of ≤0.01 (denoted by red line) for biological process (BP), cellular component (CC), and molecular function (MF) are shown, with only the top 20 biological process (BP) terms listed in (c).