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. 2023 May 22;63:102758. doi: 10.1016/j.redox.2023.102758

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© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Identification of peptide-PE adducts in an SAPE/LOX model system. Fig. 3A, negative mode C8-LC-ESI-MS chromatogram of peptide-PE adducts displaying the adduct, truncated and oxidized SAPE regions. 3A1-3, expanded m/z regions for the adduct, truncated and oxidized-SAPE regions, respectively. Oxidized SAPE species in negative mode includes m/z 782.5351 + 1[O], m/z 798.5305 + 2[O], m/z 814.5249 + 3[O], and m/z 830.5202 + 4[O]. Negative mode MS/MS analysis of the truncated peptide-PE adduct, m/z 903.43, is shown. 3 B, positive mode C8-LC-ESI-MS chromatogram of peptide-PE adducts displaying the adduct, truncated and oxidized SAPE regions. 3B1-3, expanded m/z regions for the adduct, truncated and oxidized-SAPE regions, respectively. Oxidized SAPE species in positive mode includes m/z 784.5315 + 1[O], m/z 800.5424 + 2[O], m/z 816.5376 + 3[O], and m/z 832.5328 + 4[O]. Positive mode MS/MS analysis of the corresponding truncated peptide-PE adduct, m/z 905.45, is shown.