Table 3.
Cq values and transcript quantification of the 18S rRNA and Plasmodium simium sexual antigen 25 (Pss25) targets in non-human primate samples
| Sample ID | 18S rRNA RT-qPCR | Pss25 RT-qPCR | Number of estimated gametocytes/µL# | ||
|---|---|---|---|---|---|
| Cq | Quantity (copies/µL) | Cq | Quantity (copies/µL) | ||
| J1 | 24.3 | 8.76 | 33.2 | 0.07 | 0.02 |
| J3 | 20.6 | 141.22 | – | ||
| J4 | 29.5 | 0.17 | – | ||
| J5 | 16.6 | 2,912.17 | 29.3 | 1.12 | 0.27 |
| J7 | 17.0 | 2,248.03 | 29.2 | 1.21 | 0.29 |
| J8** | 32.1 | 0.03 | |||
| J10* | 27.9 | 0.58 | – | ||
| J11 | 13.8 | 25,170.71 | 28.0 | 2.79 | 0.67 |
| J14 | 15.1 | 9,614.21 | 24.9 | 24.17 | 5.80 |
| J15 | 21.7 | 65.41 | – | ||
| J17 | 18.5 | 707.94 | 30.5 | 0.48 | 0.12 |
| J18** | 32.6 | 0.02 | |||
| J20 | 24.1 | 10.19 | 33.9 | 0.05 | 0.01 |
| J21* | 29.6 | 0.16 | – | ||
| J22 | 23.5 | 15.98 | – | ||
| J23** | 32.4 | 0.02 | – | ||
| J25 | 21.2 | 91.59 | 33.7 | 0.05 | 0.01 |
| J26 | 28.2 | 0.46 | – | ||
| J27 | 17.1 | 2,065.79 | 29.8 | 0.76 | 0.18 |
| J28 | 28.0 | 0.52 | – | ||
| J29 | 33.5 | 0.01 | – | ||
| J34 | 31.2 | 0.05 | – | ||
| J43 | 22.7 | 29.72 | – | ||
| J44 | 20.0 | 225.41 | 33.8 | 0.05 | 0.01 |
| J48 | 25.5 | 3.59 | – | ||
| C2302 | 31.6 | 0.04 | – | ||
All samples were previously detected as positive for P. simium or mixed infection, except for the indicated samples infected by P. brasilianum (*) or not infected (**). Only samples with Cq < 34 were included for quantification
#Number of gametocytes estimated according to Koepfli et al. [53]: 1 gametocyte = 4.17 Pss25 transcripts