Table 2.
Experimental design of the study
| Inputs | Negative control | Baseline control | Treatment groups | ||
|---|---|---|---|---|---|
| Group 1 | Group 2 | Group 3 | |||
| Pond water | – | 3 l | 3 l | 3 l | 3 l |
| C/N ratio | – | 15 | 15 | 15 | 15 |
| Carbon source | – | jaggery | jaggery | jaggery | jaggery |
| Nitrogen source | Feed (10%) | Ammonium sulphate + feed (5%) | Ammonium sulphate + feed (5%) | Ammonium sulphate + feed (5%) | Ammonium sulphate + feed (5%) |
| Microbial inoculum (1:1:1) | – | – | B. subtilis (AN1) + P. putida (PB3) + S. cerevisiae (ATCC-2601) | B. subtilis (AN2) + P. fluorescens (PC3) + S. cerevisiae (ATCC-2601) | B. subtilis (AN3) + P. aeruginosa (PA2) + S. cerevisiae (ATCC-2601) |
| Stocking density (800 fish m−3) | 600 nos./tank | 600 nos./tank | 600 nos./tank | 600 nos./tank | 600 nos./tank |