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. 2023 Jun 3;56:29. doi: 10.1186/s40659-023-00435-4

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — iPSCs improved the autophagy and atrophy in C2C12 myotubes under hypoxia. A Immunofluorescence staining showing MyHC expression in C2C12 myotubes after exposure to normoxia or hypoxia for 24 h with or without iPSCs co-culture. (red, MyHC; blue, DAPI). B Statistical analysis of the maximum diameter of MyHC-positive myotubes. C qPCR analysis results showing the mRNA levels of autophagy- and atrophy-related markers in C2C12 myotubes after exposure to normoxia or hypoxia for 24 h with or without iPSCs co-culture. D, E Western blot results showing the protein levels of autophagy- and atrophy-related markers in C2C12 myotubes after exposure to normoxia or hypoxia for 24 h with or without iPSCs co-culture. Statistics were from three independent replicates of the samples and data were expressed as mean ± SEM; the remaining two groups were compared with the hypoxic group, respectively, and P values are the results of one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001. ns, no significant difference. Scale bar = 100 μm. Co: iPSCs and C2C12 co-culture