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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: Mol Cancer Res. 2023 Jun 1;21(6):578–590. doi: 10.1158/1541-7786.MCR-22-0552

Fig.3.

Fig.3.

Constitutive activation of MEK-ERK signaling phosphorylates USP10 to impair its interaction with ZEB1 and thereby, stabilize ZEB1 protein. A, Analysis of ZEB1 protein expression in isogenic RKO cells expressing control shRNA or USP10 shRNA. B, Immunoblotting of K48-linkage polyubiquitination of ZEB1 in isogenic RKO cells expressing control shRNA or USP10 shRNA. C, Assessment of cell migration in isogenic RKO cells expressing control shRNA or USP10 shRNA in transwell assays and quantification of migrated cells. Migrated cells were quantified from the images (n=5) (C). Data are presented as mean ±SEM using the one-way ANOVA followed by post hoc Tukey’s test. **, p < 0.01.