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. 2023 Jun 3;14:3236. doi: 10.1038/s41467-023-39050-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Metacore pathway analysis of differentially expressed genes in injured PT clusters showing the top 10 significantly affected pathways in γGT-Cre;Tgfbr2^fl/fl compared to Tgfbr2^fl/fl 6 weeks after AA injury. b–d FACS analyses of renal leukocytes showing significant increase of CD4 + T cell number in kidneys of γGT-Cre;Tgfbr2^fl/fl mice compared to those from their Tgfbr2^fl/fl littermates 6 weeks after AA injury; n = 6 (Tgfbr2^fl/fl) and 6 (γGT-Cre;Tgfbr2^fl/fl) mice, p = 0.0085. CD8+ T cell numbers were increased in injured Tgfbr2^fl/fl compared to γGT-Cre;Tgfbr2^fl/fl kidneys, but did not reach statistical significance; n = 6 (Tgfbr2^fl/fl) and 6 (γGT-Cre;Tgfbr2^fl/fl) mice, p = 0.0984. e–h FACS analyses of renal leukocytes showing a significant increase of the number of IFNγ and TNFα producing CD4+ T cells in kidneys of γGT-Cre;Tgfbr2^fl/fl mice compared to those from their Tgfbr2^fl/fl littermates 6 weeks after AA injury; n = 6 (Tgfbr2^fl/fl) and 6 (γGT-Cre;Tgfbr2^fl/fl) mice, p = 0.0027 (IFNγ)and p = 0.0016 (TNFα). IFNγ and TNFα producing CD8 + T cell numbers were not statistically different between genotypes; n = 6 (Tgfbr2^fl/fl) and 6 (γGT-Cre;Tgfbr2^fl/fl) mice, p = 0.0519 (IFNγ) and p = 0.2419 (TNFα). i–k Cgas/Sting immunoblotting and quantification showing significantly increased expressions in renal cortices of γGT-Cre;Tgfbr2^fl/fl mice compared to those from their Tgfbr2^fl/fl littermates 6 weeks after AA injury; n = 6 (Tgfbr2^fl/fl) and 6 (γGT-Cre;Tgfbr2^fl/fl) mice, Cgas p = 0.185 and Sting p = 0.0061. α-Tubulin was used as loading and blotting control. Data are presented as mean values ± SEM. Statistical significance was determined by unpaired Student’s t test (two groups) or two-way ANOVA followed by Sidak’s multiple comparisons test, with p < 0.05 considered statistically significant. The dots represent the number of animals per group. *p < 0.05; **p < 0.01. Source data are provided as a Source data file.