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. 2023 Jun 3;14:3213. doi: 10.1038/s41467-023-38732-x

Fig. 5. Compound 5 recapitulates features of Cdk2−/ and Spdya−/ phenotypes in spermatocytes.

Fig. 5

a RAD51 (green) and SYCP3 (red) are stained in explants treated with either control or 5. b Compound 5 treated samples show significantly more localization of RAD51 at synapsed regions (positive RAD51 staining at telomeres and chromosomes) compared to control. Values graphed are mean ± SEM (n = 6 biologically independent samples for each condition). An unpaired two-tail t-test was used to determine the p-value without adjustments. c CDK2 or SPY1 (green) and SYCP1 (red) are stained in explants treated with either control or 5. White arrows added to emphasize the altered localization of RAD51/CDK2 and unchanged localization of SPY1 on chromosomes. Source data for (b) are provided as a Source Data file.