Figure 2.

Expression of Sox9 in ORS melanocytic cells

(A) Quantification of Sox9⁺/DCT⁺/GFP⁺ cells over total DCT⁺/GFP⁺ cells per HF in Ctrl mice; 7 dpi; n = 4 mice, 4–10 HF per mice; data are presented as mean ± SEM.

(B) Immunohistochemical staining of GFP (green), Sox9 (red) and DCT (white) in tamoxifen-treated Ctrl mice 7 dpi. Magnifications show signals in the ORS (B′) and hair bulb (B''); Yellow arrows point toward Sox9⁺/DCT⁺/GFP⁺ (upper panels, ORS) and yellow arrowheads point toward Sox9^neg/DCT⁺/GFP⁺ cells (lower panels, Bulb); dashed line marks the hair follicle; E = Epidermis, HF = Hair follicle, B = Bulge, ORS = Outer root sheath, Bu = Bulb; DAPI is depicted in blue (scale bar = 20 μm).