Figure 3.

Increased expression of Sox9 in immature/dedifferentiated melanocytic cells

(A) Immunohistochemical staining of GFP (green), Sox9 (red) and DCT (white) in the epidermis of tamoxifen-treated Ctrl mice 7 dpi; n = 3 mice, 2–5 20-μm-thick sections of 1 cm long mice tail skin per mice. Yellow arrowheads point toward Sox9^neg/DCT⁺ cells; dashed line marks the epidermis; E = Epidermis; DAPI is depicted in blue (scale bar = 25 μm).

(B and C) cDNA microarray (n = 3; Table S2, for details, please see Link-Paulus et al.³⁰) and (C) qRT-PCR (n = 3; normalized to β-Actin) analyzing Sox9 expression in melanoblast-related-cells (MBrc) compared to its expression in normal human epidermal melanocytes (NHEM).

(D) Representative Western Blot image, lanes were cut out of a blot image including other, irrelevant lanes (n = 3; normalized to β-Actin); (B-D) students two-sided t-test was applied to determine statistical significance (∗p< 0.05). Data are presented as mean ± SEM.