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Determination of R. solanacearum (RS)-specific crRNA targeting hrpB gene. (A) Target site and crRNA sequence of RS hrpB gene (schematic). (B) Real-time fluorescence curves of PCR/Cas12a-FQ-reporter reactions with differing crRNAs. (C) In vitro dsDNA substrate cleavage assay. No-cR: no crRNA1 in reaction mixture (negative control). CrRNA1: crRNA1 was added to reaction mixture. Red arrows: expected cleavage products.
