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. 2023 May 22;11:1188176. doi: 10.3389/fbioe.2023.1188176

FIGURE 2.

FIGURE 2

Optimization of factors in LAMP/Cas12a-fluorescence assay. (A) LbaCas12a concentration. (B) CrRNA concentration. (C) FQ-reporter concentration. (D) LAMP reaction time. NC: negative control (RNase-free water as template). Data shown are mean ± SD (n = 3). Differing letters above bars indicate significant (p < 0.05) differences by Tukey’s multiple comparison test.