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. 2023 May 22;11:1188176. doi: 10.3389/fbioe.2023.1188176

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Copyright © 2023 Fan, Mei, Xing, Chen, Hu, Liu, Li, Liu, Liu and Liang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sensitivity and specificity of LAMP/Cas12a-fluorescence assay. (A) Real-time LAMP/Cas12a-fluorescence detection of 10-fold serial dilution of pUC57-hrpB. (B) Endpoint of LAMP/Cas12a-fluorescence assay in above experiment. (C) Sensitivity of hrpB gene detection by RT-qPCR. Horizontal dashed line: threshold for determination of Ct value. (D) Detection of various strains by LAMP/Cas12a-fluorescence assay, with pUC57-hrpB as positive control template, and DEPC water as negative control (NC) template. Horizontal dashed line: fluorescence cutoff defined by average NC intensity plus three times SD. RS1-7: Ralstonia solanacearum phylotype Ⅰ; Ab: Acinetobacter baumannii; Ehs: Enterobacter hormaechei subsp; Ec: Enterobacter cancerogenus; Rr: Rhizobium rhizogenes; At: Agrobacterium tumefaciens; Xc: Xanthomonas campestris; Rp: Ralstonia pickettii; W: RNase-free water; E: empty tube. Statistical notations as in Figure 2.