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. 2023 May 22;13:1178576. doi: 10.3389/fcimb.2023.1178576

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Copyright © 2023 Ogbuigwe, Roberts, Knox, Heiser, Pita, Haack, Garcia-Ramirez, Velathanthiri, Biggs, French and Hayman

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sporo-Glo™-positive COLO-680N cells are detectable in increasing proportions with increasing multiplicity of infection (MOI) for C. parvum and C. hominis. (A) COLO-680N cells were infected at the indicated MOI (dark blue dots) with either C. parvum (top two panels) or C. hominis (bottom two panels) and then fixed, permeabilized, and stained with Sporo-Glo™. Uninfected cells stained with Sporo-Glo™ (light gray dots) are overlaid on each panel. A region defining Sporo-Glo™-positive cells is shown, and the percentage of cells in each region is displayed. (B) Triplicates from independent cultures from the experiment shown in (A) plotted as percent Sporo-Glo™ cells.