Figure EV3. Oviposition preference assays on vitamin C.

1. Photograph of egg‐laying plate to show the differentiating behavior of females in the presence of 50 mM vitamin C. Higher number of eggs were seen on the side of 50 mM vitamin C than 1 mM.
2. Oviposition preference assays of specific GRN‐ablated flies and control with the indicated concentrations of vitamin C, n = 6.
3. Oviposition preference assays with Ir25a ² , Ir76b ¹ , Gr5a ^∆5 , Gr61a ¹ , Gr64b ^LexA , Gr64c ^LexA , and Gr64e ^LexA , n = 6–7.
4. Oviposition preference assays to rescue Ir25a ² and Ir76b ¹ defects by expression of wild‐type cDNA, Ir25a ⁺ and Ir76b ⁺, driven by its own GAL4s and Gr64f‐GAL4, n = 6.
5. Rescue of the oviposition defects of Gr5a ^∆5 , Gr61a ¹ , Gr64b ^LexA , Gr64c ^LexA , and Gr64e ^LexA using GAL4/UAS system with the indicated GAL4s, n = 6. +/− indicate the presence or absence of the transgene, respectively.

Data information: All error bars represent the SEM. Multiple sets of data were compared using single‐factor ANOVA coupled with the Scheffe's post hoc test. Asterisks indicate statistical significance compared with the control. **P < 0.01.