Figure EV2. Slp and Tll expression during terminal differentiation in ectopic NBs generated via Dpn overexpression.

• A
  Time‐line depicting age of ectopic neuroblasts (NBs). Pupal formation occurs at 120 h AEL; pupae are dissected at 126 h (6 h APF), 130 h (10 h APF), and 136 h (16 h APF).
• B–D″
  Representative images of the deep medulla neuronal layer in the pupal optic lobe, in which UAS‐dpn is driven by eyR16F10‐GAL4 (outlined) and stained with the stem cell marker, Miranda (Mira, magenta), mid‐tTF, Sloppy‐paired (Slp, grey), and late tTF, Tailless (Tll, cyan). Dedifferentiated NBs express Slp and do not express Tll at 6 h APF and 10 h APF; however, they by 16 h APF they do not express Slp and instead express Tll. Quantified in (E).
• E
  Quantification of % Slp⁺ or Tll⁺ cells in Mira‐positive cells within the eyR16F10‐GAL4 expression domain in UAS‐dpn (calculated as the ratio of Slp/Tll⁺ cell volume and Mira⁺ volume within eyR16F10‐GAL4 domain). Six hours APF (Slp n = 6, m = 8.999 ± 1.64, Tll n = 6, m = 0.8718, ±0.3451). Ten hours APF (Slp n = 5, m = 5.876, ±1.752, Tll n = 5, m = 0.89 ± 0.1915). Sixteen hours APF (Slp n = 7, m = 0.4699, ±0.116, Tll n = 7, m = 3.767 ± 0.8893).
• F
  Heat shock regime for (G–G‴). Clones were induced by heat shock (red) at 48 h AEL and dissected at 126 h AEL (6 h APF), corresponding to 82 h (blue) hours after heat shock.
• G′–G‴
  Are magnified images of (G). At 82 h after clone induction (10 h APF), Mira⁺ NBs express Slp and not Tll.

Data information: Data are represented as mean ± SEM. P‐values were obtained performing unpaired t‐test, and Welch's correction was applied in case of unequal variances. **P < 0.005, *P < 0.05. Scale bars: 50 μm.