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. 2023 Apr 17;24(6):e56849. doi: 10.15252/embr.202356849

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© 2023 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Co‐immunoprecipitation (Co‐IP) in Agrobacterium tumefaciens. A. tumefaciens C58 ∆tde1 harboring pTrc200 vector or its derivatives expressing HA‐tagged Tde1 variants. Anti‐HA resin was used to co‐precipitate the Tde1 variants and Tap1.

Secretion assay for HA‐tagged Tde1 variants. Western blot for the cellular and extracellular fractions of A. tumefaciens C58 ∆tdei and ∆tdei∆tssK expressing the HA‐tagged Tde1 variants. Hcp secretion was detected as a positive control for active T6SS secretion. Representative western blot results of three biological repeats were shown with antibody against HA, Hcp, or EF‐Tu where EF‐Tu serves as a loading and nonsecreted protein control. Protein markers are indicated in kDa.

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