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. 2023 Apr 12;24(6):e56128. doi: 10.15252/embr.202256128

Figure 2. Renal tubular specific G9a deletion enhanced lipolysis and fatty acid oxidation after I/R 3D injury.

Figure 2

  • A
    Top 15 KEGG pathways enriched from significantly downregulated genes between injured kidney vs. non‐injured kidney of WT mice (left), and from significantly upregulated genes between injured kidney of Ehmt2 Ksp mice vs. injured kidney of WT mice (right), at I/R 3D; red bars indicate pathways related to lipid metabolism.
  • B, C
    Heatmap of RNA‐sequencing data (B) and qPCR validation (C) of indicated genes related to fatty acid metabolism of WT + NI (n = 6), Ehmt2 Ksp  + NI (n = 6), WT + I/R 3D (n = 6) and Ehmt2 Ksp  + I/R 3D (n = 6) groups.
  • D, E
    Heatmap of RNA‐sequencing data (D) and qPCR validation (E) of indicated genes related to fatty acid beta‐oxidation of WT + NI (n = 6), Ehmt2 Ksp  + NI (n = 6), WT + I/R 3D (n = 6) and Ehmt2 Ksp  + I/R 3D (n = 6) groups.
  • F
    Representative Cpt1a immunostaining (up) with quantitative analysis (bottom) in the kidneys of WT + NI (n = 5), Ehmt2 Ksp  + NI (n = 5), WT + I/R 3D (n = 5) and Ehmt2 Ksp  + I/R 3D (n = 5) groups. Scale bar = 50 μm. IOD, integral optical density.
  • G
    Representative CES1 immunostaining (left) with semi‐quantitative analysis (right) of human kidneys. AKI (acute kidney injury), n = 3; Con (control), n = 6. Scale bar = 50 μm.
  • H
    Representative immunoblots (left) with quantitative results (right) of Ces1 level in the kidneys of WT (n = 3) and Ehmt2 Ksp (n = 3) mice at I/R 3D.
  • I–K
    Carboxylesterase enzymatic activity (I), representative Oil Red O staining with quantification (J, scale bar = 100 μm), as well as the levels of TG (triglyceride; K, left) and TC (cholesterol; K, right) in the kidney of WT (n = 6) and Ehmt2 Ksp (n = 4) mice at I/R 3D.

Data information: NI, non‐injured; in (C, E–K), data were presented as means ± SD. Panels C (except Ces1f), E (except Acox1 and Pgc1a) and F were analyzed by 1‐way ANOVA followed by Tukey's test. Panels G–J, K(TG) were analyzed with 2‐tailed, unpaired Student's t test. Panels C (Ces1f), E (Acox1 and Pgc1a) and K (TC) were analyzed by Mann–Whitney U test. *P < 0.05; **P < 0.01.

Source data are available online for this figure.