Figure EV4. Phenotypic surveys for the clones from a new batch of suppressor development including the wild‐type controls.

1. The “desiccation hypersensitivity” phenotypic spectrum of 20 each 28°C‐evolved new batch clones (one clone of the new batch MATα 28°C‐sef1Δ‐Evo was removed from the collection due to contamination). The desiccation sensitivities of each clone are displayed according to the qualitative viabilities (Dataset EV16) as described above. The sef1Δ background seems more prone to generate adaptive mutations with desiccation hypersensitivity (18/19 in MATα and 17/20 in MATa lines) than the wild‐type background (3/20).
2. The “Dex‐trade‐off” phenotypic spectrum of 20 each 39°C‐evolved new batch clones. The “Dex‐trade‐off” phenotypes of each clone are displayed according to the simple fitness scores under the YPD_37°C condition (Dataset EV16) as described above. Clones with score 1 (with fitness worse than sef1Δ strain) were defined as “with Dex‐trade‐off” for the new batch 28°C‐sef1Δ‐Evo while a score ≤ 3 (with fitness worse than the wild‐type strain) for the 28°C‐WT (wild‐type)‐Evo. About 14.74% (23/156) of clones in this batch showed no “Dex‐trade‐off.” Unlike observed in the first batch of suppressor development, there is no clear preference in any genetic background to generate a higher frequency of adaptive mutations with “Dex‐trade‐off” (5/20 in the wild‐type, 2/20 in MATα, and 3/20 in MATa lines).

Data information: For (A) and (B), the dots labeled with colors (red, green, or orange in different lines) stand for clones isolated from the same population in each line while the dots labeled with black from different populations.