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. 2023 Apr 3;24(6):e56019. doi: 10.15252/embr.202256019

Figure 5. Phenotypic response to azf1Δ mutation in the YPGly condition.

Figure 5

  1. Selected GO terms enriched under the YPGly condition. The adjusted P‐values of the hypergeometric test with Bonferroni correction are shown.
  2. The counteracting effect of hypomorphic hsf1 on growth in response to azf1Δ mutation in both wild‐type and sef1Δ backgrounds. HSF1 is an essential gene in yeast (Solís Eric et al, 2016), so a hypomorphic hsf1 mutation with a truncated C‐terminus (460–557 amino acids removed; Sorger, 1990) rather than a null mutant was used in L. kluyveri strains.
  3. Partial compensation of defective TCA cycle gene expression in sef1Δ mutants by azf1Δ mutation under the YPGly condition. The heatmap was generated using the mean TPM ratio from RNA‐seq data relative to the sef1Δ strain under each condition.
  4. Transcriptional remodeling of the core glycerol utilization and auxiliary pathways in response to azf1Δ mutation under the YPGly condition. The heatmap was generated using the mean TPM ratio from RNA‐seq data relative to the wild‐type under each condition. The high‐resolution source tables of the heatmaps are provided in Dataset EV17.